Traditional Dioxin Cleanup System

The PrepLinc Traditional Dioxin Cleanup System automates the multi-step Dioxin Cleanup procedure.  PrepLinc SPEi Column modules allow flow from column to column.  Elutions can flow through, bypass or reverse elute through any column module.  Fractions can be collected or transferred to the AccuVap for concentration.  


Adding the AccuVap allows sample to be collected between steps so sorbent amounts can be decreased and columns are less expensive.  The traditional method of Silica, Alumina and Carbon cleanup with the reverse elution of dioxin analytes to the AccuVap for inital concentration is completely automated with this configuration.

Method Compatibility

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Cartridge compaitiblity

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Concentrate Between Steps

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Reverse Elution

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Positive Pressure

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Method Programming

This very complex process for cleanup of samples for analysis of PCBs, PBDEs, PCDDs and PCDFs can be accomplished in two SPEi methods using the PrepLinc™ software.


Method 1 is a 3-column method that injects the sample through the Silica (SPM1) and Alumina (SPM 2) columns to the AccuVap™.  An elution flows through Silica & Alumina to the AccuVap.  A second fraction flows through all three columns to the AccuVap.  A third fraction flows through Alumina & Carbon to the AccuVap.  And an optional 4th fraction flows just through the carbon column to the AccuVap. The fractions is held in the chamber to be combined with the next elution.  This fraction contains all PCB’s and PBDE’s.


Method 1 could be split into two methods if it is desired to analyze any of the first 4 fractions separately.


Method 2 is a single column method in which Toluene is reverse eluted through the Carbon (SPM 3) column and sent to the AccuVap™ for concentration.  The concentrated fraction is exchanged and transferred to a separate vial for analysis of PCDDs and PCDFs.


The Linc Method for this process creates one multi-step method out of the two SPEi methods.  In the Sequence screen, the tray and SPM column positions for the first sample are defined and as more samples are added to the sequence the positions for additional samples are automatically indexed.  Programming for a very complex sample prep process just got simple!

Modified POPs Prep System

The PrepLinc™ Modified Dioxin and Persistent Organic Pollutants Cleanup System  Adding the GPC Cleanup module gives another cleanup technique option so sample prep  can be customized for unique matrix and analyte situations.  Adding the AccuVap™ allows sample to be concentrated between steps so sorbent amounts can be decreased and columns are less expensive.  

Adding GPC Cleanup

The PrepLinc™ GPC Module with Direct Inject gives the option to use this bulk cleanup step to replace or 

enhance other traditional cleanup steps specific to different matrices.

Method Example

Using the Linc Editor in the software, four different methods involving different modules can be linked to run together as one method.  


For this application we have a methods to (1) Condition the alumina cartridge; (2) GPC Cleanup to AccuVap™ and out to SPE cartridge; (3) Elution through the same SPE cartridge to AccuVap™ and collected in a vial; and (4)Elution through the same SPE cartridge with a different solvent to AccuVap™ and collected in a different vial.